Optimizing Tangential Flow Filtration
Optimizing Tangential Flow Filtration
Empowering Precise, Low-Shear Purification and Buffer Exchange
In downstream bioprocessing, Tangential Flow Filtration (TFF), also known as cross-flow filtration, is a critical technique for concentrating, desalting, and buffer exchanging biomolecules such as proteins, viruses, and other biologics—minimizing fouling and enhancing efficiency through parallel flow across the membrane. This process separates contaminants like DNA fragments, ions, or cell debris from feedstock, supporting high-purity production of biosimilars, vaccines, cell and gene therapies, monoclonal antibodies (mAbs), and recombinant proteins.
CPC Biotech solutions deliver gentle, stable flow control, non-invasive monitoring, and aseptic connections, ensuring optimalrecirculation rates (cross-flow) and trans-membrane pressure(TMP) for stable dynamics, reduced particle buildup, and highfiltration rates without fouling.
Engineered for single-use systems and sterile operations, these tools address challenges like shear-sensitive biologics, variable feed properties, and frequent changeovers in multi-product facilities. By providing low-pulsation transfers, precise metering, and quick integrations, we optimize TFF workflows for batch and continuous processing—reducing energy use, validation burdens, and costs while achieving higher yields, regulatory compliance, and faster time-to-market.
6 Key Benefits of our TFF Solutions
- Superior Purity and Recovery:
Low-shear handling preserves biologic integrity, minimizing denaturation and product damage for high yields in protein purification and virus concentration.
- Minimized Contamination Risk:
Closed, aseptic designs with gender less connectors eliminate microbial ingress, maintaining flow path sterility during media transfers and buffer exchanges.
- Enhanced Efficiency:
Stable, low-pulsation flow at high pressure supports optimal TMP control, enabling shorter runs, seamless scale-up, and up to 50% less energy consumption—less energy equals less heat input, less shear stress, and higher product yields.
- Precision Control:
Non-invasive sensors provide real-time, accurate measurements unaffected by flow regime or velocity variations, ensuring consistent cross-flow for fouling prevention.
- Scalability and Flexibility:
Self-priming pumps with high turndown ratios and versatile connectors facilitate quick changeovers, multi-product campaigns, and integration with single-use filter cassettes.
- Regulatory Compliance:
USP Class VI materials, gamma-sterilizable options, and rigorous testing (materials validation, product testing, torture-to-failure) deliver extractables reports and sterility assurance for patient safety.
Biopharma manufacturers rely on our purpose-built tools to advance TFF for cell harvesting, buffer dilution, and purification—delivering reliable, high-throughput results while handling increased cell densities and diverse therapeutic modalities.
Quattroflow® Precision Pumps for Gentle TFF Flow Control
Quattroflow quaternary diaphragm pumps are engineered for gentle, stable flow control in TFF, featuring a four-piston design that requires no mechanical seals or wetted rotating parts—ensuring total product containment with minimal risk of shear, pulsation, and particulate generation. Self-priming with high turndown ratios, they maintain consistent linear flow for optimal buffer-dilution performance, supporting precise recirculation and TMP management in sterile, single-use setups.
Best Practices for Optimal Performance
In TFF setups, Quattroflow pumps enable consistent low-shear operation essential for stable processes, reducing energy needs and preserving product integrity during concentration and buffer exchange. Pair with BioPro sensors for accurate volume tracking, ensuring precise control over cross-flow rates in sterile environments.
Advanced Flow Sensors for Accurate TFF Monitoring
Non-invasive flow measurement is vital for TFF, delivering real-time precision on recirculation rates and TMP without media contact—critical for hygienic transfers and fouling prevention in single-use lines.
Ultrasonic BioPro™ Flow Meters (Powered by em-tec)
BioPro Sensors use ultrasonic transit-time technology for highly accurate, calibration-free measurement with no obstructions in the flow path, ideal for single-use buffer-dilution in TFF. USP Class VI compliant and gamma-stable, they provide integrated air/bubble detection for air-sensitive biologics.
Optimal Solution
For single-use TFF applications due to ease of use and reliability, BioPro Sensors ensure non-invasive measurement critical for transferring hygienic liquids, enabling data-driven TMP adjustments for optimal filtration rates.
The Best Options for Tangential Flow Filtration
Coriolis SumoFlo® Flow Meters
SumoFlo Flow Meters offer best-in-class ±1% flow-rate accuracy unaffectedby flow regime or velocity profile variations, with additional density andtemperature data for precise buffer dilution in TFF. Compact, gamma-sterilizable (USP Class VI) and excel in viscous or bubbly fluids.
Optimal Solution
With precise, accurate measurement for bufferdilution, SumoFlo Flow Meters ensure successful TFF performance regardless of fluid properties, minimizing disruptions and enhancing biologic yields.
Connectors for TFF Integration
Sterile genderless connectors provide instant sterility for media transfers in tangential flow filtration including robust seals, excellent flow-to-size ratios, compatibility with steam, gamma irradiation, and various terminations (hose barbs and tri-clamps). Validated for extractables, they optimize workflows for high-purity purification.
- Features: Sizes range from 1/8" to 1.5" and withstand actuation stress – enable multiple configurations for modularity in single-use assemblies.
- Benefits: Connections in seconds with no particulates, eliminating contamination risk and providing connection assurance for efficient changeovers.
- Applications: Aseptic linking of TFF modules to bioreactors and holdingbags in downstream buffer exchange and concentration.
CPC Biotech vs the Competition
Quattroflow Four Piston Diaphragm Pumps
Engineered for gentle, stable flow control, Quattroflow Four-Piston Diaphragm Pumps maintain consistent linear flow for optimal buffer-dilution performance.
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SumoFlo Coriolis
Flow Meters
With best-in-class accuracy, SumoFlo Coriolis Flow Meters ensure precise, accurate measurement for buffer dilution.
BioPro FlowSU
System
Ultrasonic BioPro FlowSU Systems are optimal for single-use buffer-dilution applications due to their ease of use and reliability.
BioPro Clamp-On
Sensors
Ideal for monitoring flow rate in laboratory buffer-dilution applications, BioPro Clamp-On sensors rely on non-invasive measurement technology.
Connect With Our Experts
CPC Biotech offers a comprehensive range of flow control solutions that can be tailored to meet your specific bioprocessing requirements.
Fill out the form below to connect with an expert and get started.
2820 Cleveland Ave. N.,
Roseville, MN 55113, USA
Phone: (+1) 651-645-0091
Toll-Free: 1-800-444-2474
info@cpc-bio.com
© 2026 CPC Biotech. ALL RIGHTS RESERVED.
© 2026 CPC Biotech. ALL RIGHTS RESERVED.